Update: 28 September, 2023
This document describes the method of rapid protein characterization using microflow LC/MS/MS techniques. The method involves digestion of a protein followed by microflow LC separation of the peptide fragments. Each peptide fragment is automatically selected for collision induced dissociation (CID) using mass spectrometric techniques, including automated data-dependent scan functions. Protein identification is achieved by correlating uninterpreted protein and peptide tandem mass spectral data with sequences in protein or nucleotide databases using computer algorithms. This method offers the potential for automated high-throughput data analysis and provides a high degree of specificity to database searches due to the unique fingerprint-type pattern displayed by the tandem mass spectrum representing every amino acid sequence.
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MD5 Checksum: 0C8DBA29FE5D99139974432114BE8F6E
Publication date: 02 July, 2012
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PDF Link: Agilent Rapid Protein Characterization using Microflow LC/MS/MS Techniques PDF