Agilent Efficient peptide fractionation improved protein identification with the Agilent 3100 OFFGEL Fractionator

Update: 29 September, 2023

This document illustrates the effectiveness of the Agilent 3100 OFFGEL Fractionator for the prefractionation of complex protein or peptide mixtures. The tryptic digest of E. coli proteins was fractionated into 24 fractions, and peptides were identified using reversed phase liquid chromatography with mass spectrometric detection. The distribution of peptides and their isoelectric points in each fraction was analyzed. The results show efficient peptide fractionation and improved protein identification using the Agilent 3100 OFFGEL Fractionator.

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MD5 Checksum: 85FAE600CFBE0296CA199FE3D6413039

Publication date: 02 July, 2012

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