Agilent Characterization of Protein Phosphorylation Using HPLC-Chip Electron Transfer Dissociation Ion Trap Mass Spectrometry

Update: 29 September, 2023

This note describes the characteristics and importance of protein phosphorylation, as well as the method of qualitative analysis of protein phosphorylation using HPLC-Chip electron transfer dissociation ion trap mass spectrometry technology. By comparing the differences between collision-induced dissociation (CID) and electron transfer dissociation (ETD) mass spectrometry techniques, it demonstrates the ability to analyze samples using both CID and ETD in the same LC/MS run. Examples of the identification of site-specific phosphorylation using an Agilent HPLC-Chip system are provided.

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Publication date: 02 July, 2012

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