Update: 30 September, 2023
Quantitative proteomics using multiple reaction monitoring (MRM) has emerged as an important methodology for biomarker validation. Multiple-reaction monitoring on a triple quadruple (QQQ) mass spectrometer provides superior sensitivity and selec- tivity for targeted peptides in a complex sample. MRM also offers high precision in quantitation and a fast scan speed, which makes it an ideal technology for validating biomarkers in a high-throughput fashion. This application note demonstrates the entire workflow for biomarker validation offered by Agilent Technologies using multiple reaction monitoring together with statistical analysis software. Thousands of peptide transitions were generated using Agilent Q-TOF and Spectrum Mill MRM selector. The transition list was imported into MassHunter acquisition software equipped with dynamic MRM functionality and monitored in a single LC/MS run. Finally, MRM quantitation results were analyzed using Mass Profiler Pro for principle component analysis (PCA), hierarchical clustering and ANOVA analysis.
File format: PDF
Size: -
MD5 Checksum: 5436A6034B0A8D658B596106CC57C94F
Publication date: 02 July, 2012
Downloads: -
PDF Link: Agilent High-Throughput Protein Quantitation Using Multiple Reaction Monitoring Application Note PDF