Update: 30 September, 2023
A profile-directed biomarker discovery approach involving initial differential expression analysis followed by targeted identification of differentially expressed proteins was used in this study. Using Agilent platforms such as a Multiple Affinity Removal (MARS) column, a 1200 Series HPLC-Chip/MS and a 6520 Accurate-Mass Quadrupole Time-of-Flight (Q-TOF) LC/MS, excellent reproducibility in retention time and intensity measurement, and outstanding accuracy in mass measurement and sensitivity over a wide dynamic range were achieved. Differential analyses using MassHunter Mass Profiler and MassHunter Mass Profiler Professional (MPP) software enabled the comparison between spiked and non-spiked plasma samples. Subsequent targeted MS/MS analysis on a Q-TOF LC/MS followed by a database search using Spectrum Mill helped identify the spiked protein.
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MD5 Checksum: 4C325C0777D366F605A0BDD6D83B0BE7
Publication date: 02 July, 2012
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