Update: 30 September, 2023
This Application Note describes the development of a rapid resolution LC method for the separation of the Chinese herbal prescription Zuo Jin Pill into its six major con- stituents: magnoflorine, jatrorrhizine, coptisine, palmatine, berberine and evodiamine. The method uses an Agilent 1200 Series Rapid Resolution Liquid Chromatography (RRLC) system coupled with an Agilent 1200 Series Diode Array Detector (DAD). The separation was carried out on an Agilent ZORBAX Eclipse Plus C18 column (4.6 × 150 mm, 1.8 µm). The linear gradient was eluted with the mobile phase of an acetoni- trile – acetate buffer. All calibration curves showed good linearity (r2 >0.9998) within the test range 1.2-1200 µg/ml. The results demonstrate that this analytical method is simple, sensitive and reliable for rapidly analyzing six major bioactive compounds in ZJPs. It is useful for the comprehensive evaluation of the quality of this traditional Chinese Medicine (TCM).
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MD5 Checksum: FA05867F176DA198B47ADC4C792B8F89
Publication date: 02 July, 2012
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