Update: 30 September, 2023
This application note describes a rapid method for the quantification of total testosterone in serum using the Agilent 1200 Series Rapid Resolution LC (RRLC) system with the Agilent 6430 Triple Quadrupole LC/MS in atmospheric pressure chemical ionization (APCI) mode. A two dimensional LC approach was used to minimize matrix interferences and ion suppression, while reducing the time required for analysis. Excellent linearity of quantification (R2 > 0.99) was obtained in the range of 5 to 2000 ng/dL, with analysis times of only 4.5 minutes, and day-to-day precision was below 10%. The limit of detection (LOD) and limit of quantification (LOQ) were 2 ng/dL and 5 ng/dL respectively, in 5% bovine serum albumin.
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MD5 Checksum: 89DBE5C5209715375290E7697ADF4874
Publication date: 02 July, 2012
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PDF Link: AGILENT Analysis of Testosterone in Serum using LC/Triple Quadrupole MS/MS Application Note PDF