Update: 29 September, 2023
This article describes the analysis of saponins in spine date seed using Agilent ZORBAX Solvent Saver HT (1.8 µm) columns. The two main active compounds, Jujuboside A and B, were extracted and analyzed by high performance liquid chromatography (HPLC) with Agilent ZORBAX Eclipse Plus C18 LC columns. The method was first developed on a traditional 4.6 mm × 150 mm, 5 µm column and then transferred to a ZORBAX Eclipse Plus Solvent Saver High Throughput (1.8 µm) C18 column, 3.0 mm × 50 mm, 1.8 µm. The advantages of the Solvent Saver HT column include low solvent consumption and faster analysis. An evaporative light scattering detector (ELSD) was used due to the lack of chromophores in the two compounds. This provided higher sensitivity and a cleaner baseline compared with UV detection.
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Publication date: 12 June, 2012
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