Update: 30 September, 2023
The document introduces a method for the determination of 5-MOP and 8-MOP in lemon oil and orange oil using the Agilent 1290 Infinity LC system. At 1000 bar, the total analysis time in reversed-phase (RP) liquid chromatography (LC) with acetonitrile and water as mobile phase components could be reduced to 4 min. In the framework of green chromatography, and the present ace- tonitrile shortage, mobile phases constituted of water/methanol and water/ethanol were compared to water/acetonitrile. The influence of the nature of organic modifiers on selectivity and peak capacity was investigated. The figures of merit of the different methods were compared for the determination of 5-MOP in a lemon oil sample con- taining approximately 50 mg/kg. 8-MOP was not detected at the ppm level. The detection limit for both furocoumarins was approximately 1 mg/kg citrus oil.
File format: PDF
Size: -
MD5 Checksum: 9B1690650FB3C814E810C69A4F84B664
Publication date: 12 June, 2012
Downloads: -