Update: 01 October, 2023
Two isocratic reversed-phase methods that have distinct selectivity, and use popular UV/MS or UV detection, are presented for analyzing Rebaudioside A and other steviol glycosides. A Rapid Resolution High Throughput (RRHT) Eclipse Plus Phenyl-Hexyl method using UV and MS detection resolved many compounds from a Stevia rebaudi- ana Bertoni plant extract instead of the other approach employing an amino column with a refractive index (RI) or other special detector. The RRHT Eclipse Plus Phenyl- Hexyl method’s advantages include high peak capacity, isocratic mobile phase, low acetonitrile consumption, and unique selectivity compared to a ZORBAX Carbohydrate Analysis column method. Three commercially available Stevia sweeteners were ana- lyzed for Rebaudioside A and Stevioside, the two major diterpenoid glycosides present in stevia leaves.
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MD5 Checksum: AB334FC5E9E36CDC5D90B82D74C39237
Publication date: 12 June, 2012
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PDF Link: Agilent Isocratic Stevia Sweetener Analysis using Selective ZORBAX Columns Manual PDF