Update: 30 September, 2023
A high performance liquid chromatography tandem mass spectrometric (HPLC/MS/MS) method was developed for the simultaneous determination of 10 antibiotic residues: minocycline, 4-epioxytetracycline, 4-epitetracycline, tetracycline, 4-epichlortetracycline, demeclocycline, chlortetracycline, methacycline, doxycycline, oxytetracycline in milk and animal tissues. In the method, Agilent’s novel solid phase extraction cartridge and a reversed phase Agilent ZORBAX RX C8 column (5 µm, 150 mm × 2.1 mm) were used for purification and separation. The limit of detection (LOD) is between 0.5 and 10.0 µg/kg and the limit of quantitation (LOQ) is less than 50 µg/kg. The linearity is obtained from 5 to 1000 µg/kg. Overall recoveries are between 76.4% and 101% with a relative standard deviation (RSD, n = 6) less than 8.4%. The method is rapid, sensitive, convenient and robust, and can be used to simultaneously confirm multi-residues of tetracyclines and their metabolites in milk.
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Publication date: 12 June, 2012
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