Update: 30 September, 2023
This application note describes the use of Agilent ZORBAX Eclipse Plus C18, Eclipse Plus Phenyl-Hexyl, Eclipse XDB-Phenyl and StableBond SB-Phenyl Rapid Resolution columns to analyze nine common food additives using an isocratic solvent system. The mobile phases used include methanol or acetonitrile with 20 mM phosphate buffer at pH 2.5 or 0.2 percent formic acid. While excellent peak shape is found on all columns examined, selectivity and resolution differences are found when comparing C18 and various phenyl columns, especially the Eclipse Plus Phenyl-Hexyl and StableBond SB-Phenyl columns. In addi- tion, selectivity differences and peak shape are preserved even when using a formic acid mobile phase modifier. The use of formic acid would allow verification of unknown materials using mass spectrometry.
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MD5 Checksum: D5BEFF9086AAE4A13575FDE5EC48B1AA
Publication date: 12 June, 2012
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