Agilent Development of an LC-MS/MS Method for the Determination of 20-Hydroxyecdysone Its Metabolites in Calf Urine Manual

Update: 29 September, 2023

This article describes the development of an LC-MS/MS method for the determination of 20-hydroxyecdysone and its metabolites in calf urine, and its application to the control of potential misuse in cattle. The method involves purification on two solid-phase extraction cartridges (SPE C18 and SPE SiOH) and LC-(ESI+)-MS/MS measurements. The sensitivity and specificity of tandem quadrupole MS/MS allowed measurements at trace levels in both spiked and incurred samples. Good linearity was observed for all analytes from 0.12 ng to 12 ng on column.

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Publication date: 12 June, 2012

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