Update: 30 September, 2023
This application note describes methods for analyzing the levels of melamine and cyanuric acid in pet food and related raw materials using the Agilent 7890A-5975C GC/MS and 1200SL-6410 Triple Quadrupole LC/MS. The GC/MS method employs extraction and then trimethylsilyl derivatization and is used here for screening. The detection limit is 10 µg/g. The LC/MS/MS method requires only extraction and then a simple isocratic elution on a normal-phase silica column (Rx-Sil) with water and acetonitrile mobile phase containing 5 mM ammonium acetate. The separation of the two compounds takes 5 min. The linear range is from 50 pg/mL to 50 ng/mL for melamine and from 1 ng/mL to 100 ng/mL for cyanuric acid. The LC/MS/MS method is demonstrated by analysis of the target compounds in complex food matrix.
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Publication date: 12 June, 2012
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