Agilent Improving the Effectiveness of Method Translation for Fast High Resolution Separations Manual

Update: 29 September, 2023

The increased availability of sub-2-micron (STM) columns and increased demand for methods friendly to mass spectrometers has led to a strong trend toward conversion of existing HPLC methods to smaller diameter and smaller particle size columns. While the conversion is a simple mathematical exercise requiring the scaling flow rates, gradient times, and injection volumes, many users observe less than perfect results. Here we look closely at the problem and propose calculations that improve the speed and/or resolution in a more predictable and beneficial way.

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File format: PDF

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MD5 Checksum: 6C1EFBA5B37A2C41C8D0FD73F14D655F

Publication date: 12 June, 2012

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PDF Link: Agilent Improving the Effectiveness of Method Translation for Fast High Resolution Separations Manual PDF