Update: 30 September, 2023
This paper describes the configuration and operation of a combined liquid chromatography/mass spectrometry (LC/MS) system for the identification and isolation of DSP-toxins. In the analytical mode, okadaic acid (OA) and dinophysistoxin-1 (DTX-1) are more selectively and sensitively monitored when compared to LC with fluorescence detection. With less sample preparation, the detection limits are decreased by a factor of 3–5, depending on the matrix. In semipreparative mode, OA and DTX-1 could be isolated from crude extracts of Prorocentrum lima algae using mass-based fraction collection with a purity >98%. Due to this method, reference standards of DSP toxins are now commercially available.
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Publication date: 12 June, 2012
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