Agilent The HPLC Preparative Scale-Up of Soybean Phospholipids Manual

Update: 28 September, 2023

This document describes a method for separating major classes of soybean phospholipids from a crude mixture using a matched set of columns, normal phase conditions, and a small amount of volatile salt added to the mobile phase, under both analytical and preparative conditions. However, at higher concentrations of phosphatidylcholine, two distinct peaks were observed, whose areas changed with the mass injected. Mass spectrometry confirmed that these two peaks are the same or very similar.

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Publication date: 12 June, 2012

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PDF Link: Agilent The HPLC Preparative Scale-Up of Soybean Phospholipids Manual PDF