Update: 30 September, 2023
This study analyzed synthetic dyes using ion-pair, reversed phase chromatography on a special base-deactivated HPLC column. This separation mechanism was chosen to reduce tailing effects of highly polar colors. Detection was performed using a new design of diode-array detector based on two lamps — a deuterium lamp and a tungsten lamp. This ensured highest light output at 190 – 950 nm, which resulted in lowest detection limits over the entire wavelength range. It was possible to analyze blue, black or green colors in the low ng range at their absorption maxima of 600 – 700 nm. Complete spectra, feasible from 190 – 950 nm, facilitated identification with an automated library search.
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MD5 Checksum: 8A0FD2DA3F6D7A6B8ED7E00374CA3125
Publication date: 12 June, 2012
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