Update: 28 September, 2023
This article describes the analysis of synthetic dyes in food samples using capillary zone electrophoresis with an alkaline phosphate buffer as the background electrolyte. The precision of the method was found to have a relative standard deviation of less than 0.5% for the run-to-run migration times and 2% for the peak areas with buffer replenishment after each run. The detection limit for individual dyes was approximately 1 ng using a 50-µm internal diameter Agilent extended path length capillary. Compounds were detected at different wavelengths (215, 410, 520, and 598 nm) and the identities of the individual dyes were confirmed using peak-purity routines and a UV-Visible spectral library.
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MD5 Checksum: 3DD2B8B90A704C158DD358C8540103F7
Publication date: 12 June, 2012
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PDF Link: Agilent Analysis of Synthetic Dyes in Food Samples by Capillary Zone Electrophoresis Manual PDF