Update: 28 September, 2023
Several oxidizable vitamins (vitamin A, B6, C, D3 and E) were analyzed using HPLC and electrochemical detection. Excellent sensitivity was achieved with all vitamins. The minimum detectable level for vitamin A- palmitate was about 80 pg, for β-carotene about 50 pg, for vitamin B6 about 30 pg, for vitamin C below 1 pg, for vitamin D3 about 70 pg and for vitamin E about 30 pg. The optimum potentials were evaluated using an automated increment for the potentials. The reproducibility was tested using vitamin B6 and was found to be 3% RSD of peak height for 100 runs. Due to the high selectivity of electrochemical detection, it was possible to keep sample preparation very simple.
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Publication date: 12 June, 2012
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PDF Link: Agilent Analysis of Selected Vitamins with HPLC Electrochemical Detection Manual PDF