Update: 30 September, 2023
This study used an Agilent HPLC-Chip coupled with an Agilent Accurate-Mass Q-TOF LC/MS to analyze the glycopeptide and glycan moiety of a monoclonal antibody (mAb). The tryptic peptide map of the mAb was generated and the glycopeptide was assigned using accurate mass measurement. The enzymatically released glycan moiety from the mAb was extracted and analyzed on an HPLC-Chip coupled with a Q-TOF. Diagnostic oxonium ions from a glycan moiety were further used to identify the glycopeptide and the glycan. The superior mass accuracy of the LC/MS platform, combined with the powerful data processing capabilities of Agilent MassHunter and BioConfirm software, enabled identification of the glycopeptides and glycan moiety attached to the protein complex.
File format: PDF
Size: -
MD5 Checksum: E7BE8553D55676AF035844DB6882C147
Publication date: 12 June, 2012
Downloads: -