Update: 30 September, 2023
This application note describes a sensitive, precise, and accurate quantitative liquid chromatography/tandem mass spectrometry (LC/MS/MS) method for the measurement of beta-estradiol acetate in human plasma. The sample preparation method employed solid phase extraction on Agilent SampliQ cartridges. The processed samples were chromatographed on an Agilent ZORBAX Eclipse Plus C18 analytical column and analyzed by triple-quadrupole tandem mass spectrometry in the multiple reaction monitoring (MRM) mode using negative electrospray ionization. The principal advantage of the LC/MS/MS method described in this application note is the simultaneous achievement of high absolute recovery (~ 80%), high sensi- tivity (LLOQ of 100 fg injected on-column or 28.6 pg/mL plasma), high precision (≤ 5.9% CV) and accuracy (81.5-118.1%), as well as excellent linearity over the concentration range 0.1 to 10000 pg/μL (R2 ≥ 0.998) with a short run time of only 1.2 min.
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MD5 Checksum: E8EF004CD5D585D1742378F8257053C3
Publication date: 12 June, 2012
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