Agilent Automated MRM Method Optimizer for Peptides: Optimizing Mass Spectrometry Parameters for High-Throughput Protein Quantitation Application Note

Update: 28 September, 2023

This document introduces an automated MRM method optimizer for peptide protein quantitation. The MassHunter Optimizer software is used to optimize collision energies for 40 MRM transitions in LC/MS/MS analysis and compare them with collision energies from Q-TOF experiments. Optimized collision energies result in up to a two-fold increase in sensitivity and signal-to-noise ratio of MRM peaks. The MassHunter Optimizer software helps reduce the time and sample required for optimization by up to 200-fold.

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MD5 Checksum: 04C9FF54E2C3A448C5BE832B3C056194

Publication date: 12 June, 2012

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PDF Link: Agilent Automated MRM Method Optimizer for Peptides: Optimizing Mass Spectrometry Parameters for High-Throughput Protein Quantitation Application Note PDF