Agilent Rapid Method Assessment of Nonsteroidal Anti-Inflammatory Separations using Agilent ZORBAX Rapid Resolution High Throughput Columns with the Agilent 1200 Series Method Development Solution Con

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Two samples consisting of various nonsteroidal anti-inflammatory drugs (NSAID) are separated using methods developed on an Agilent 1200 Series Method Development Solution controlled by ACD/Labs AutoChrom for ChemStation software. The chromatograph while under control of AutoChrom can screen up to seven columns, thirteen buffers and two organic solvents with columns held in four temperature controlled zones. The software helps plan the next best experiment to perform, allowing the analyst to focus on quickly developing methods using conditions with the best likelihood of success. In this work four columns (Agilent ZORBAX StableBond SB-C18, Agilent ZORBAX Eclipse Plus C18, Agilent ZORBAX Bonus RP and Agilent ZORBAX Eclipse Plus Phenyl-Hexyl) are screened using five mobile phase modifiers. Twenty solvent column experiments are initially screened for each sample set.


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Publication date: 11 June, 2012

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