Agilent The Influence of Silica Pore Size on Efficiency Resolution Loading in Reversed-Phase HPLC Manual

Update: 30 September, 2023

This paper presents the influence of the silica pore size on the separation, efficiency and capacity of a separation using a number of standard compounds. The study uses a prep to process material, SepTech, which uses ultra pure silica as the base particle and a bonding chemistry to give a high carbon load C18 material. Two pore sizes, a 60Å (ST60 10-C18) and a 150Å (ST150 10- C18) were used. The results show that the silica pore size has a large impact on the separation efficiency, resolution and capacity.

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Publication date: 11 June, 2012

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PDF Link: Agilent The Influence of Silica Pore Size on Efficiency Resolution Loading in Reversed-Phase HPLC Manual PDF