Agilent Flow cytometric analysis of human primary cells using the Agilent 2100 bioanalyzer on-chip staining Manual

Update: 28 September, 2023

This article describes a method for flow cytometric analysis of human primary cells using the Agilent 2100 bioanalyzer and on-chip staining. The transfection efficiency of primary cells can be determined using green fluorescent protein (GFP) as a reporter molecule. The advantages of using the compact 2100 bioanalyzer for monitoring transfection efficiency in primary cells include low cell consumption, high reproducibility of results, fast on-chip staining procedure, and ease of use.

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Publication date: 11 June, 2012

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