Update: 28 September, 2023
This document describes the enhanced analysis of semi-volatile compounds using sub-ambient ELSD and HPLC. Evaporative light scattering detection (ELSD) is a powerful tool that can detect any sample that is less volatile than the mobile phase in HPLC applications, regardless of the optical properties of the compounds. ELSD offers distinct advantages over conventional UV or DAD detection, especially for gradient separations. In order to maximize the detection of non-UV absorbing semi-volatile analytes, ELSD detectors need to operate at lower temperatures. Most ELSDs today can evaporate highly aqueous eluents at ambient temperature, but for low molecular weight, low boiling point compounds, ambient temperature is still too high and sensitivity is poor. Sub-ambient ELSD is required to improve the detection of these compounds. The Agilent 385-ELSD is specifically designed to operate under these demanding conditions. It features a Peltier cooled evaporation tube that provides sub-ambient ELS detection down to 10 °C. Dry nitrogen gas is added during the evaporation step to facilitate solvent removal at sub-ambient temperatures.
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Publication date: 11 June, 2012
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PDF Link: Agilent Enhanced Analysis of Semi-volatile Compounds using Sub-ambient ELSD HPLC handbook PDF