Update: 01 October, 2023
This application note describes how the Agilent 2100 bioanalyzer can be used in combination with Standardized RT (StaRT)-PCR reagents from Gene Express National Enterprises (G.E.N.E.), Inc. (Huntsville, AL, USA) to obtain highly reproducible, standardized gene expression measurement with RT-PCR. The internal standards used in StaRT-PCR control for variable loading of PCR products into electrophoresis chan- nel, and allow quantitative gene expression measurement at the end- point of PCR, without real-time analysis. Gene expression measure- ment of 15 genes was more reproducible with the Agilent 2100 bioana- lyzer than with the ABI Prism 310 Genetic Analyzer or agarose gel elec- trophoresis methods. The mean coefficient of variance (CV) for these three methods was 0.26, 0.39 and 0.50, respectively.
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MD5 Checksum: 348D0C79A03096900454A32E80785992
Publication date: 11 June, 2012
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