Update: 30 September, 2023
This Application Note describes a simplified and fast protocol for the detection of apoptotic cells with the Agilent 2100 bioanalyzer and the cell fluorescence LabChip®. The specific advantage of the simplified protocol is the amount of time saved and its ease-of-use. The note describes how the Agilent 2100 bioanalyzer can be used to analyze apoptotic cell samples. Annexin V staining of live cells is demonstrated and the data are compared to that obtained with a conventional flow cytometer. Histogram quality of the microfluidic system is comparable to that of a conventional flow cytometer. Detailed protocols and reagent recommendations for staining of cells are provided. The high reproducibility of the chip results, low cell and reagent consumption, the speed and the ease of use are advantages the Agilent 2100 bioanalyzer offers for monitoring apoptotic cells by annexinV staining.
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MD5 Checksum: D14C3BB78B0A6BAC62BD7FF1735CE9CD
Publication date: 11 June, 2012
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