Update: 28 September, 2023
This Application Note describes how the Agilent 2100 bioanalyzer and the cell fluorescence LabChip® kit can be used to determine the efficiency of transfection of mammalian cells using green fluorescent protein (GFP) as a reporter molecule by on-chip staining. Transfection of CHO-K1 cells with an expression vector encoding GFP was performed and cells were stained on-chip with the live-dye CBNF (carboxynaphthofluorescein). The histogram quality and the percent transfected cells determined are in good agreement with data obtained using a conventional flow cytometer. Detailed protocols and reagent recommendations for analyzing transfection reactions are provided. Speed of the on-chip staining procedure, high reproducibility of the chip results, low cell consumption and ease-of-use are advantages the Agilent 2100 bioanalyzer offers for monitoring transfection efficiency using GFP.
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MD5 Checksum: 0D601413E1FAC19C3BF09E1AE7ADF3DF
Publication date: 11 June, 2012
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PDF Link: Agilent Monitoring transfection efficiency in cells using an on-chip staining protocol Manual PDF