Update: 01 October, 2023
This Application Note demonstrates that the combination of HPLC with the Agilent 385-ELSD Evaporative Light Scattering Detector provides a better alternative for the sensitive analysis of polar lipids. Evaporative light scattering detection is universal and not dependent on the optical properties of the compound, making it ideal for analysis of lipids such as cholesterol and phosphatidylcholine that have very poor UV chromophores. ELS detection recognizes any compound that is less volatile than the mobile phase. This makes ELS detection fully compatible with HPLC elution gradients. ELS detection also eliminates the need for pre-analysis derivatization to enhance UV absorbance, facilitating rapid determination of lipids in complex matrices.
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Publication date: 11 June, 2012
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