Update: 30 September, 2023
This application note details a quick and effective analytical method for the determination of low and trace level polycyclic aromatic hydrocarbons (PAHs) in fish samples as an alternative to procedures involving more time consuming and complex techniques. A simplified QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) method with dispersive solid phase extraction (dSPE) provided sufficient sample matrix cleanup while preserving low level analyte detection. A pressure controlled tee was installed post column to implement the use of automated backflush to diminish residual sample carryover and reduce instrument cycle times. The Agilent J&W DB-5ms Ultra Inert (UI) 20 m × 0.18 mm , 0.18 µm column effectively resolved the 16 targeted PAHs. A GC/MS method employing selective ion monitoring (SIM) was calibrated at the 10, 25, 50, 100, 250, 500, and 1000 ng/mL PAH levels, yielding excellent linearity and reproducibility. The spiked levels for recovery determinations were 25, 250, and 500 ng/mL. Recoveries ranged from 80% and 139% with RSD below 6%.
File format: PDF
Size: -
MD5 Checksum: 50B6550B55051BF905675EC602B6FD46
Publication date: 11 June, 2012
Downloads: -