Update: 28 September, 2023
This application note demonstrates sensitive and reproducible HPLC analysis of N-linked glycans of human immunoglobulin G using the Agilent 1260 Infinity Bio-inert Quaternary LC System with fluorescence detection. The precision of retention times and areas was determined for analysis of a N-linked human IgG glycan library. In addition, good linearity in a range from 0.008 to 1 pmol was determined for a mix of five glycan standards (Man5, G2, G2S1, G2FS1, and G2S2) with low limits of detection and quantification. The Agilent 1260 Infinity Bio-inert Quaternary LC System with fluorescence detection is an optimal system for sensitive and reproducible analysis of 2-AB derivatized N-glycans released from human immunoglobulin G.
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MD5 Checksum: 3A7E6EDA41AD846B1C17B335E9F0B34B
Publication date: 11 June, 2012
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PDF Link: Agilent Sensitive reproducible glycan analysis of human immunoglobulin G Manual PDF