Agilent LC/MS/MS of Vitamin B Shows Effects of Injection Solvents with ZORBAX HILIC Plus Manual

Update: 30 September, 2023

This application note describes the optimization of a rapid LC/MS/MS method for vitamin B related compounds (4-aminobenzoic acid, nicotinamide, riboflavin, and nicotinic acid) on an Agilent ZORBAX Rapid Resolution High Definition HILIC Plus column using an Agilent 1290 Infinity LC System with an Agilent 6410A Triple Quadrupole Mass Spectrometer. The method uses isocratic elution with an acetonitrile rich mobile phase and an ammonium acetate buffer. All analytes have good peak shape with this hydrophilic interaction chromatography (HILIC) application. The method is then used to demonstrate the importance of choosing an appropriate injection solvent when performing LC analyses in HILIC mode. Various injection solvents are investigated, including water, acetonitrile, methanol, and combinations. Of the solvents explored, it was determined that pure acetonitrile yields the best retention and peak shape for all compounds, with water being the worst solvent for analytical performance and peak shape.

Brand: Agilent

File format: PDF

Size: 692 KB

MD5 Checksum: 5295A1CD90B0C3027396E28F753E3181

Publication date: 08 June, 2012

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PDF Link: Agilent LC/MS/MS of Vitamin B Shows Effects of Injection Solvents with ZORBAX HILIC Plus Manual PDF

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