Agilent Optimizing Performance of an Agilent ZORBAX RRHD Eclipse Plus C18 Column by Enhancing an Agilent 1290 Infinity LC System for Ultra-Low Dispersion Manual
Update: 29 September, 2023
An Agilent 1290 Infinity LC System is optimized for the lowest possible extra-column volume, to ensure minimal band broadening and optimal results from a small dimension, Agilent ZORBAX RRHD Eclipse Plus C18, 2.1 × 50 mm, 1.8 µm column. Improvements to isocratic and gradient analyses are evident, with the early eluting peaks from the isocratic analysis showing the greatest improvement of up to a 58% increase in efficiency (k’ = 1.6). Gradient analyses are improved by 24 to 32% with respect to conditional peak capacity (k’ = 1.3 to 5.3). Additionally, there is no strong correlation between flow rate and the effect of extra-column volume, with respect to column performance for isocratic and gradient analyses.
Brand: Agilent
File format: PDF
Size: 823 KB
MD5 Checksum: 6C1C07930DF43BDF4FC980CACF2B2ED7
Publication date: 08 June, 2012
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