Agilent Transfer of a USP method for prednisolone from normal phase HPLC to SFC using the Agilent 1260 Infinity Hybrid SFC/UHPLC System Manual

Update: 29 September, 2023

This Application Note describes the transfer of a United States Pharmacopeia (USP) method for prednisolone from normal phase HPLC to SFC using the Agilent 1260 Infinity Hybrid SFC/UHPLC System. The results show that the SFC method meets the system suitability criteria, is 4x faster, and results in 17x lower solvent expenses. Robustness tests on the SFC method demonstrate excellent robustness for routine analysis.

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Publication date: 08 June, 2012

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PDF Link: Agilent Transfer of a USP method for prednisolone from normal phase HPLC to SFC using the Agilent 1260 Infinity Hybrid SFC/UHPLC System Manual PDF