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- Last added
- 2026-10-03
Featured HaiGene manuals
Haigene 1000U RNase Free DNase I Product Leaflet
The RNase Free DNase I enzyme randomly cleaves single‑ or double‑stranded DNA, generating 5′‑phosphate oligonucleotides. Protease is almost completely removed, improving stability at neutral pH, and the formulation contains an RNase inhibitor to protect RNA during extraction.
The leaflet provides component quantities, activity definition, purity data, storage conditions, and a step‑by‑step protocol for removing DNA contamination from RNA samples, including buffer preparation, incubation, and heat inactivation steps. This manual is written in Chinese.
Covers: Description · Components · Activity Definition · Purity · Applications · Storage
Haigene LAMP Operation Guide
The document provides a historical background of the LAMP technique, describing its development by Notomi and subsequent improvements by Nagamine. It outlines the principle of using six specific primers and a strand‑displacing DNA polymerase to achieve rapid, high‑efficiency nucleic acid amplification at a constant temperature.
Practical sections detail primer design rules, recommended reaction components, and step‑by‑step protocols for both DNA‑LAMP and RNA‑RT‑LAMP using Haigene's Bst polymerases. Various detection methods, including visual precipitation, SYBR Green I fluorescence, and colorimetric dyes, are also described. This manual is written in Chinese.
Covers: Historical Background · Primer Design Principles · DNA LAMP Amplification Procedure · RNA RT‑LAMP Amplification Procedure · Detection and Colorimetric Methods
Latest HaiGene manuals
Haigene 1000U RNase Free DNase I Product Leaflet
Product Leaflet 1 pages · 167 KB · ZHThe Haigene 1000U RNase Free DNase I enzyme degrades DNA while preserving RNA, includes RNase inhibitor and detailed usage instructions.
Haigene LAMP Operation Guide
User Manual 4 pages · 388 KB · ZHThis guide explains the principles, primer design, reaction setup, and detection methods for Haigene's loop-mediated isothermal amplification (LAMP) assays.
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in HaiGene manuals
What defines one activity unit of this DNase I?
One unit is the amount of enzyme that completely digests 1 μg of pBR322 plasmid DNA in a 50 μl reaction at 37 °C for 10 minutes.
How much enzyme should be used for RNA samples containing more than 5 μg of genomic DNA?
Use 2 μl of the enzyme for samples with over 5 μg of genomic DNA contamination.
What are the recommended melting temperatures for LAMP primers?
F1c/B1c should be 64–65 °C, F2/B2 59–61 °C, F3/B3 59–61 °C, and LoopF/LoopB 64–65 °C.
How can LAMP products be detected visually?
By observing the white precipitate of magnesium pyrophosphate formed during the reaction.