STRATAGENE Manuals (Health)

Our next generation Brilliant II® QPCR and QRT-PCR Master Mix Kits offer superior sensitivity for improved quantification and reproducibility. Our new Brilliant® II QPCR and QRT-PCR reagents were developed to maintain the high quality of our original Brilliant product line, but with a significant improvement in sensitivity of detection, ensuring reproducible quantification even at low target concentrations.

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DNA Marker product introduction

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Alien® QRT-PCR Inhibitor Alert is a high-quality external control for detecting inhibitors in RNA samples for QRT-PCR experiments.

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The Brilliant II and Brilliant II FAST SYBR QPCR Master Mixes are single-tube reagents designed for performing quantitative PCR amplifications with an accelerated cycling protocol that completes 40 cycles of PCR in approximately 48 minutes. The master mix formulations and fast cycling protocol were developed together to create a system for completing QPCR reactions in less time without compromising target detection sensitivity, specificity, or reproducibility. The Brilliant II QPCR Master Mixes have been successfully used with fluorescent TaqMan probes and SYBR Green to amplify and detect a variety of DNA targets, including genomic DNA, plasmid DNA, and cDNA. The Brilliant II FAST QPCR kit includes 2× master mix containing hot start Taq DNA polymerase, dNTPs, Mg2+, and a buffer specially formulated for fast cycling. The Brilliant II FAST SYBR QPCR Master Mix also includes the DNA binding dye SYBR Green for detection. The DNA polymerase features a rapid hot start capability that reduces nonspecific product formation while keeping the run time of the PCR protocol to a minimum, making it ideal for the Brilliant II QPCR kits. Both Brilliant II FAST master mixes have been developed, tested, and validated on our Mx3000P and Mx3005P QPCR systems. By utilizing our Mx QPCR systems in combination with our new Brilliant II FAST QPCR kits

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AccuScript™ High Fidelity Reverse Transcriptase products are the best reverse transcriptase for first-strand cDNA synthesis, producing the highest transcription accuracy possible. It is the ideal choice for any application that requires premium error-free cDNA.

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AffinityScript ™ Multiple Temperature Reverse Transcriptase is an engineered enzyme with high affinity for primer template complexes and can synthesize cDNA across a broad range of temperatures from 37°C to 55°C. The enzyme promotes cDNA yield due to its high affinity for primer:template complexes.

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SideStep ™ II QRT-PCR products allow you to skip RNA purification and go directly to QRT-PCR

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The SpotReport® Alien® Array Validation System* is a group of controls that collectively validates microarray data. They can be used for any genome microarray and play a pivotal role in normalization, monitoring hybridization specificity, and checking overall data quality from each microarray.

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This document describes a rapid method for detecting bacteria using PCR. The method can detect eight of the most common species of bacteria in as little as three hours and can avoid false positive and false negative results.

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