Agilent Technologies High precision restriction fragment sizing with the Agilent 2100 Bioanalyzer Application Note

Update: 30 September, 2023

This application note describes how the DNA 12000 LabChip® kit can be used with the Agilent Technologies 2100 bioanalyzer to size DNA fragments generated by restriction enzyme digestion. Sizing accuracy was found to be greater than 92 % for all samples analyzed. Compari- son of the chip-based separations to those obtained using agarose gel electrophoresis indicates that the DNA 12000 assay accurately sizes fragments over a wider range than can be usually analyzed on a slab gel.

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Publication date: 03 July, 2012

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PDF Link: Agilent Technologies High precision restriction fragment sizing with the Agilent 2100 Bioanalyzer Application Note PDF