Key facts
- Manuals
- 2
- Categories
- 1
- Languages
- ZH
- Last added
- 2000-01-01
Featured DNA manuals
TaKaRa D6137 DNA Fragmentation Kit User Manual
This manual describes the DNA Fragmentation Kit, which enables random fragmentation of genomic DNA without the need for ultrasonic devices. The kit includes enzymes, buffers, and solutions required for fragmentation and subsequent end‑repair, allowing direct ligation to adapters.
The document details the preparation of reaction mixtures, thermal cycler settings, and handling precautions. It also includes example protocols, troubleshooting Q&A, and guidelines for storage and component handling. This manual is written in Chinese.
Covers: Kit Contents · Storage · Precautions · Operation Procedure · DNA Fragmentation Protocol · End Repair Protocol
Promega DC6700/DC6701 DNA IQTM System Small Sample Case Manual
This manual describes the components of the DNA IQTM System and provides step‑by‑step procedures for extracting DNA from solid‑material stains and liquid samples. It includes preparation of lysis and wash buffers, magnetic resin handling, heating steps, and elution.
The document also offers a troubleshooting guide, detailed buffer compositions, and a list of related Promega products such as MagneSphere® magnetic stands and PowerPlex® kits. This manual is written in Chinese.
Covers: Introduction · Product Components · DNA IQTM System Operation Steps · Troubleshooting · Buffer and Solution Composition · Related Products
Latest DNA manuals
TaKaRa D6137 DNA Fragmentation Kit User Manual
User Manual 2 pages · 471 KB · ZHThe TaKaRa D6137 DNA Fragmentation Kit User Manual provides step‑by‑step instructions for DNA fragmentation and end‑repair procedures.
Promega DC6700/DC6701 DNA IQTM System Small Sample Case Manual
User Manual 8 pages · 529 KB · ZHChinese operation manual for Promega DNA IQTM System (DC6700/DC6701) covering small‑sample DNA extraction and troubleshooting.
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in DNA manuals
Why are DNA fragments too small after fragmentation?
Enzyme-1 is highly temperature‑sensitive; all steps must be performed on ice and the reaction tube should not be touched to avoid temperature rise that over‑fragments DNA.
Why is DNA fragmentation insufficient?
Insufficient mixing of the reaction mixture can lead to incomplete fragmentation; ensure thorough mixing during steps A‑3 and B‑2.
What is the DNA binding capacity of the resin?
The resin can capture up to about 100 ng of DNA per reaction.
From: Promega DC6700/DC6701 DNA IQTM System Small Sample Case Manual
What temperature is used for the heating step during DNA extraction?
The sample is heated at 95 °C for 30 minutes.
From: Promega DC6700/DC6701 DNA IQTM System Small Sample Case Manual