DNA Fragmentation Kit is a random fragmentation DNA kit produced by TaKaRa Company. This kit can fragment long-chain DNA such as genomic DNA without using ultrasonic disintegrators or other special instruments under the action of enzymes, and smooth the ends of DNA fragments. DNA fragments processed with this kit can be directly linked to smooth-ended vectors. If it is not necessary to smooth the ends, the reaction can be terminated after the DNA fragmentation reaction. This kit is also suitable for the concentration of methylated DNA and the pretreatment of rapid DNA sequencing.
This manual describes the DNA Fragmentation Kit, which enables random fragmentation of genomic DNA without the need for ultrasonic devices. The kit includes enzymes, buffers, and solutions required for fragmentation and subsequent end‑repair, allowing direct ligation to adapters.
The document details the preparation of reaction mixtures, thermal cycler settings, and handling precautions. It also includes example protocols, troubleshooting Q&A, and guidelines for storage and component handling. This manual is written in Chinese.
| Enzyme-1 amount | 20 µL |
|---|---|
| Dilution Buffer-1 amount | 1.040 µL |
| Stop solution amount | 400 µL |
| Recommended DNA input (low amount) | ≤100 ng for 10 µL reaction |
Enzyme-1 is highly temperature‑sensitive; all steps must be performed on ice and the reaction tube should not be touched to avoid temperature rise that over‑fragments DNA.
Insufficient mixing of the reaction mixture can lead to incomplete fragmentation; ensure thorough mixing during steps A‑3 and B‑2.