This assay kit is for research use only and is designed for the detection of rat bone alkaline phosphatase (BALP) using enzyme-linked immunosorbent assay (ELISA). The kit has high specificity and can simultaneously detect native or recombinant rat BALP without cross-reactivity with other related proteins. The detection range is from 15.6 U/L to 1000 U/L, with a minimum detection limit of 3.9 U/L. The kit has a shelf life of 6 months. It is intended for quantitative measurement of BALP levels in rat serum, plasma, cell culture supernatant, or other relevant biological fluids.