This kit is for research use only and is intended for quantitative measurement of laminin-332 (LN) in rat serum, plasma, cell culture supernatant or other relevant liquids by ELISA. The kit uses a double antibody sandwich enzyme immunoassay to detect the level of laminin-332 in the specimen. Purified antibody is coated on a micro-plate to form a solid phase antibody. The micro-well of the coated single antibody is sequentially added with laminin-332, biotinylated anti-rat laminin-332 antibody, HRP-labeled affinity, and then thoroughly washed with the substrate TMB for color development. TMB is converted to blue under the catalysis of peroxidase and finally converted to yellow under the action of acid. The color depth is positively correlated with the laminin-332 content in the sample. The absorbance (OD value) is measured at 450nm wavelength by the enzyme label, and the sample concentration is calculated.
