This kit is for research use only and is intended for quantitative measurement of laminin-332 (LN) in rat serum, plasma, cell culture supernatant or other relevant liquids by ELISA. The kit uses a double antibody sandwich enzyme immunoassay to detect the level of laminin-332 in the specimen. Purified antibody is coated on a micro-plate to form a solid phase antibody. The micro-well of the coated single antibody is sequentially added with laminin-332, biotinylated anti-rat laminin-332 antibody, HRP-labeled affinity, and then thoroughly washed with the substrate TMB for color development. TMB is converted to blue under the catalysis of peroxidase and finally converted to yellow under the action of acid. The color depth is positively correlated with the laminin-332 content in the sample. The absorbance (OD value) is measured at 450nm wavelength by the enzyme label, and the sample concentration is calculated.
This manual describes the ELISA sandwich assay for quantifying rat laminin in serum, plasma, cell culture supernatants, or other specimens. It details the composition of the kit, preparation of standards and reagents, and the step‑by‑step protocol for sample incubation, washing, substrate development, and measurement at 450 nm.
Guidelines for sample collection, storage conditions, washing procedures, and calculation of concentrations are provided, along with safety notes, recommended incubation times, and detection limits of the assay. This manual is written in Chinese.
| Detection range | 1.56 ng/ml – 100 ng/ml |
|---|---|
| Standard stock concentration | 100 ng/ml |
| Storage temperature for reagents | -20 °C for some reagents; 2–8 °C for others |
The kit can detect laminin concentrations from 1.56 ng/ml up to 100 ng/ml.
Serum, plasma, or cell culture supernatants should be kept at –20 °C and avoid repeated freeze‑thaw cycles.
Add 50 µl of 2 N H₂SO₄ to each well to terminate the reaction, turning the color from blue to yellow.