This document is the instruction manual for the Rat Type I Procollagen (PCⅠ) ELISA Kit, which is used for quantitative measurement of the content of PCⅠ in rat tissue homogenates, serum, plasma, cell culture supernatants, or other related fluids. The experimental principle involves coating the microplate with purified antibodies to create a solid-phase carrier, and sequentially adding the specimen or standard, biotinylated anti-rat PCⅠ antibody, and HRP-labeled streptavidin. After thorough washing, the substrate TMB is used for color development. The color intensity is positively correlated with the PCⅠ content in the sample. The absorbance (OD value) is measured at a wavelength of 450nm using an ELISA reader to calculate the sample concentration.
