Chinese operation manual for Promega DNA IQTM System (DC6700/DC6701) covering small‑sample DNA extraction and troubleshooting.
This manual describes the components of the DNA IQTM System and provides step‑by‑step procedures for extracting DNA from solid‑material stains and liquid samples. It includes preparation of lysis and wash buffers, magnetic resin handling, heating steps, and elution.
The document also offers a troubleshooting guide, detailed buffer compositions, and a list of related Promega products such as MagneSphere® magnetic stands and PowerPlex® kits. This manual is written in Chinese.
| Reactions per kit | 400 |
|---|---|
| Resin DNA binding capacity | ≈100 ng |
| Elution buffer composition | 10 mM Tris (pH 8.0) + 0.1 mM EDTA |
| Heating step for lysis | 95 °C for 30 min |
The resin can capture up to about 100 ng of DNA per reaction.
The sample is heated at 95 °C for 30 minutes.
The elution buffer contains 10 mM Tris (pH 8.0) and 0.1 mM EDTA.