Key facts
- Manuals
- 1
- Categories
- 1
- Languages
- ZH
- Last added
- 2012-01-11
Featured rat胎盘生长因子(PLGF)酶联免疫分析试剂盒 manuals
Rat Placental Growth Factor ELISA Kit User Manual
This manual describes the ELISA method for quantitatively measuring rat placental growth factor (PLGF) in various biological fluids. It explains the principle of using a coated micro‑plate with capture antibody, biotinylated detection antibody, and HRP‑streptavidin, followed by a TMB substrate reaction measured at 450 nm.
The kit includes a 96‑well plate, standards, sample diluent, detection reagents, substrate, wash buffer, and stop solution. Detailed steps cover sample preparation, reagent handling, incubation times (typically 37 °C for 60–120 min), washing procedures, color development, and data analysis using a standard curve. This manual is written in Chinese.
Covers: Principle of the ELISA · Reagents and Preparation · Sample Collection and Storage · Assay Procedure · Washing Steps · Data Analysis and Standard Curve
Latest rat胎盘生长因子(PLGF)酶联免疫分析试剂盒 manuals
Rat Placental Growth Factor ELISA Kit User Manual
User Manual 5 pages · 119 KB · ZHInstructions for using the rat placental growth factor ELISA kit to quantify PLGF in serum, plasma, cell culture supernatants, and other biological samples.
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in rat胎盘生长因子(PLGF)酶联免疫分析试剂盒 manuals
What is the detection range of the kit?
The kit can detect PLGF concentrations from 3.12 pg/mL up to 200 pg/mL.
At what wavelength should the absorbance be measured?
Absorbance should be read at 450 nm.