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Featured rat睾酮(T)酶联免疫吸附测定试剂盒 manuals
USCN E0458R Rat Testosterone ELISA Kit User Manual
This kit uses a competitive inhibition ELISA to quantitatively determine testosterone levels in rat serum. The assay relies on competition between the sample testosterone and a labeled antigen for binding to a specific antibody coated on a 96‑well plate. After incubation and washing, the substrate TM B produces a color change inversely proportional to the testosterone concentration, which is measured at 450 nm.
The manual lists all reagents (96‑well plate, wash buffer, standards, TM B substrate, detection solutions A and B, stop solution) and required equipment (plate reader with 450 ± 10 nm filter, pipettes, EP tubes, distilled water, absorbent paper). It provides detailed steps for sample preparation, reagent preparation, plate loading, incubation, washing, color development, termination, and data analysis, along with storage conditions, shelf life, and safety warnings. This manual is written in Chinese.
Covers: Principle of the assay · Reagents and materials · Sample collection and storage · Assay procedure · Washing steps · Data analysis and calculation
Latest rat睾酮(T)酶联免疫吸附测定试剂盒 manuals
USCN E0458R Rat Testosterone ELISA Kit User Manual
User Manual 4 pages · 267 KB · ZHThe USCN E0458R manual explains how to measure rat testosterone in serum using a competitive ELISA assay.
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in rat睾酮(T)酶联免疫吸附测定试剂盒 manuals
What is the detection range of the rat testosterone ELISA kit?
The assay can detect testosterone concentrations from 0.08 to 20 ng/mL.
How should the standard solutions be prepared?
Dilute the concentrate with 285 mL of distilled water to make 300 mL of 20‑fold wash buffer, and prepare standards at 20, 5, 1.25, 0.31, 0.08, and 0 ng/mL.