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Featured rat瘦素(LEP)酶联免疫吸附测定试剂盒使用说明书 manuals
USCNK E0084Ra Rat Leptin ELISA Kit User Manual
This manual describes the ELISA method for measuring rat leptin (LEP) in serum, plasma, cell culture supernatants, and tissue extracts. It lists the required reagents, plate format, and the principle of the assay, which uses a 96‑well plate coated with anti‑LEP antibodies and a biotin‑labeled detection antibody.
The document provides detailed procedures for sample preparation, reagent preparation, plate washing, incubation steps, and color development with TMB substrate. It also includes guidance on reading absorbance at 450 nm, calculating concentrations using standard curves, and storage conditions for reagents and plates. This manual is written in Chinese.
Covers: Principle of the ELISA · Sample collection and storage · Reagents and preparation · Assay procedure · Data analysis and calculation · Storage conditions
Latest rat瘦素(LEP)酶联免疫吸附测定试剂盒使用说明书 manuals
USCNK E0084Ra Rat Leptin ELISA Kit User Manual
User Manual 5 pages · 423 KB · ZHInstructions for using the USCNK rat leptin ELISA kit, covering reagents, assay steps, and data analysis.
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in rat瘦素(LEP)酶联免疫吸附测定试剂盒使用说明书 manuals
What wavelength should be used to read the ELISA results?
Read the absorbance at 450 nm.
What is the detection range of the rat leptin ELISA kit?
The assay can detect leptin concentrations from 0.312 ng/mL up to 20 ng/mL.