Key facts
- Manuals
- 2
- Models
- 1
- Languages
- ZH
- Last added
- 2012-03-14
Chaperone models by category
Featured Chaperone manuals
Takara Chaperone Competent Cells BL21 User Manual
This manual describes the Chaperone Competent Cells BL21 product, which consists of Eco BL21 cells transformed with a chaperone plasmid set. The cells are shipped and stored at -80 °C and are supplied in multiple 100 µL vials containing different chaperone plasmids.
The document details the recommended thawing, mixing, DNA addition (≤10 ng), heat‑shock steps, recovery in SOC medium, and plating procedures. It also notes incompatibility with T7‑based expression vectors, the prohibition of electroporation, and storage guidelines to maintain high transformation efficiency. This manual is written in Chinese.
Covers: Product Description · Product Contents · Plasmid Types and Inducers · Compatible Expression Plasmids · Storage · Experimental Procedure
Takara BL21 Chaperone Competent Cells User Guide
This guide describes the Chaperone Competent Cells BL21 series, including the five plasmid types (pPGro7, pKJE7, pG‑Tf2, pTf16, pKJE8) that provide chaperone assistance for improved protein solubility. It lists the components supplied, such as 100 µL cell suspensions per tube and pUC19 DNA control.
The manual provides detailed storage conditions, thawing steps, and a step‑by‑step transformation procedure, including recommended DNA amounts, heat‑shock parameters, and post‑transformation recovery. It also notes incompatibility with T7‑based expression vectors and outlines antibiotic selection guidelines for co‑expression experiments. This manual is written in Chinese.
Covers: Product Description · Product Contents · Storage · Transformation Procedure · Co‑expression Experiment · Transformation Efficiency
Latest Chaperone manuals
Takara Chaperone Competent Cells BL21 User Manual
User Manual 2 pages · 260 KB · ZHThe Takara Chaperone Competent Cells BL21 User Manual provides storage, handling, and transformation instructions for the BL21 chaperone cell kit.
Takara BL21 Chaperone Competent Cells User Guide
User Manual 2 pages · 301 KB · ZHInstructions for using Takara BL21 Chaperone Competent Cells, covering storage, transformation protocol, plasmid compatibility and safety notes.
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in Chaperone manuals
What temperature should the Chaperone Competent Cells BL21 be stored at?
They should be stored at -80 °C.
Can the BL21 cells be used with T7‑promoter expression vectors?
No, the cells do not express T7 RNA polymerase and are not compatible with T7‑based vectors such as the PET series.
How should the competent cells be stored?
Store the cells at –80 °C; if not kept at this temperature the transformation efficiency will drop.
What is the maximum amount of DNA to use for a transformation?
Use no more than 10 ng of DNA per reaction to maintain high transformation efficiency.